{
  "label": "Recomputes a binder-evidence claim: binding hashes, replicates and controls, curve fit, potency and CV, off-target selectivity, independent replication and claim honesty. It does not run an assay or prove in-vivo efficacy.",
  "public_key_b64": "HkFG0ycWJ3ys2TSn51Ho+syxZsE6FWM7KL9DFqvZ+cQ=",
  "records": [
    {
      "checks": [
        {
          "detail": "a peptide sequence over the 20 standard residues",
          "id": "binding.sequence",
          "ok": true
        },
        {
          "detail": "target 'IL-6'",
          "id": "binding.target",
          "ok": true
        },
        {
          "detail": "the protocol is named by hash",
          "id": "binding.protocol_hash",
          "ok": true
        },
        {
          "detail": "the raw data is named by hash",
          "id": "binding.raw_data_hash",
          "ok": true
        },
        {
          "detail": "3 replicate(s)",
          "id": "assay.replicates",
          "ok": true
        },
        {
          "detail": "positive 'pass'",
          "id": "assay.positive_control",
          "ok": true
        },
        {
          "detail": "negative 'pass'",
          "id": "assay.negative_control",
          "ok": true
        },
        {
          "detail": "blanks subtracted",
          "id": "assay.blanks_subtracted",
          "ok": true
        },
        {
          "detail": "R^2 0.98 >= 0.95",
          "id": "assay.curve_fit",
          "ok": true
        },
        {
          "detail": "mean Kd 12.0333 nM <= 100.0 nM",
          "id": "potency.kd_threshold",
          "ok": true
        },
        {
          "detail": "CV 3.207% <= 20.0%",
          "id": "potency.replicate_cv",
          "ok": true
        },
        {
          "detail": "the headline Kd matches the replicates",
          "id": "potency.reported_kd_matches",
          "ok": true
        },
        {
          "detail": "every off-target clears the fold",
          "id": "specificity.fold",
          "ok": true
        },
        {
          "detail": "no off-target flagged",
          "id": "specificity.no_flagged_off_target",
          "ok": true
        },
        {
          "detail": "lab 'lab-b'",
          "id": "replication.independent_lab",
          "ok": true
        },
        {
          "detail": "the same protocol was used",
          "id": "replication.same_protocol",
          "ok": true
        },
        {
          "detail": "delta 6.371% <= 20.0%",
          "id": "replication.within_tolerance",
          "ok": true
        },
        {
          "detail": "claimed True, computed True",
          "id": "claims.replicated",
          "ok": true
        },
        {
          "detail": "claimed True, computed True",
          "id": "claims.specific",
          "ok": true
        },
        {
          "detail": "claimed True, computed True",
          "id": "claims.validated",
          "ok": true
        }
      ],
      "created_at": "2026-11-12T07:59:14Z",
      "engine": "a75-012/1.0.0",
      "extra": {
        "computed": {
          "replicated": true,
          "specific": true,
          "validated": true
        },
        "cv_pct": 3.2066,
        "mean_kd_nm": 12.0333
      },
      "failed": [],
      "label": "Recomputes a binder-evidence claim: binding hashes, replicates and controls, curve fit, potency and CV, off-target selectivity, independent replication and claim honesty. It does not run an assay or prove in-vivo efficacy.",
      "prev_record_hash": "3b879b3ddc2008d414812b9b35e9306776599d5d2845f55db3ef2521e4652a6f",
      "public_key_b64": "HkFG0ycWJ3ys2TSn51Ho+syxZsE6FWM7KL9DFqvZ+cQ=",
      "record_hash": "f98e1013300cacd49fe694fa616f9838a9957e64ce6b0f3718ba3ee30ef17b06",
      "schema": "cain42.a75.binder.record.v1",
      "signature_b64": "9PNaVUdkwI5D31qsNcbm9p59AueDHx+iRTC6qECxZ9fK/Q3sfSmgUGprdxyRwsQr3+SRqu8eIIRkBDc3Q2XmDg==",
      "sku": "CAIN-A75-012",
      "subject": {
        "assay": {
          "assay_type": "SPR",
          "controls": {
            "negative": "pass",
            "positive": "pass"
          },
          "max_cv_pct": 20.0,
          "replicate_kd_nm": [
            11.5,
            12.2,
            12.4
          ]
        },
        "claim": {
          "binder_sequence": "GSTGRLQELARLLQGGP",
          "kd_threshold_nm": 100.0,
          "replication_tolerance_pct": 20.0,
          "reported_kd_nm": 12.03,
          "selectivity_fold": 10.0,
          "target": "IL-6"
        },
        "claims": {
          "replicated": true,
          "specific": true,
          "validated": true
        },
        "independent_replication": {
          "kd_nm": 12.8,
          "lab_id": "lab-b",
          "performed": true,
          "same_protocol": true
        },
        "methods": {
          "calibration_date": "2026-10-01",
          "instrument": "Biacore-8K",
          "protocol_sha256": "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa"
        },
        "off_targets": [
          {
            "flagged": false,
            "kd_nm": 450.0,
            "target": "TNF-alpha"
          },
          {
            "flagged": false,
            "kd_nm": 380.0,
            "target": "IL-1beta"
          }
        ],
        "raw_data": {
          "blanks_subtracted": true,
          "curve_fit_r2": 0.98,
          "sha256": "bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb"
        },
        "schema": "cain42.a75.binder.evidence.v1"
      },
      "subject_sha256": "c49eb82b497c599a8664df7e3e04a477a10ca6c793342788a1b49d96afdd4413",
      "verdict": "SUPPORTED"
    }
  ],
  "run_hash": "7dfdd2f4bbf265dfa78e167d808f02c8a8607748e17403f01e5c17562ce0af7e",
  "schema": "cain42.a75.binder.run.v1",
  "sku": "CAIN-A75-012",
  "verdict": "VERIFIED",
  "verdicts": [
    "SUPPORTED"
  ]
}